Generator

Part:BBa_K2068004

Designed by: Nithin Dharmaraj   Group: iGEM16_UCLA   (2016-09-13)


PCQuad Mutant 13

In this gene we have introduced a Thrombin cleavage site(LVPRGS) to our Wild Type PCQuad 3.0 gene(BBa_K2068000). This gene will function exactly like the PCQuad 3.0 as it will code for a single subunit of a self assembling 12 subunit protein cage. But, the subunits that form will include a Thrombin cleavage site and the resulting cage will be susceptible to controlled disassembly using a Thrombin protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 408
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 768


[edit]
Categories
//awards/part_collection/2016
Parameters
None